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NCNM clinic lab can get the serum IFA test for Trypanosoma Cruzi Antibodies from Quest for $247.00, tests for both IgM and IgG. I'd like to find out more about inexpensive salivary tests. According to my reading, one must run two different assays for antibodies, as the different strains of the disease express differently, but Quest doesn't seem to recognize this. If the two tests contradict each other, there is a tiebreaker third to order. Not sure where to order all these, have read parts from the links behind the cut.

blood donor testing
http://onlinelibrary.wiley.com/doi/10.1046/j.1537-2995.1995.35395184278.x/full

seropositivy in US
negligible prevalence of ab's in blood donors in southeast
http://www.ncbi.nlm.nih.gov/pubmed/9353087
in blood donors in 2006-7
http://www.medscape.com/viewarticle/552928
in cardiac pts: http://circ.ahajournals.org/cgi/content/full/102/24/2978

seropositivity in Brazil
http://www.ncbi.nlm.nih.gov/pubmed/9163992
2..66% in Buenos Aires
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B7CPT-4BM4R39-4B&_user=2488959&_coverDate=12%2F31%2F2001&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000057540&_version=1&_urlVersion=0&_userid=2488959&md5=d3893e2ea6547fe065eabecb97cec38a&searchtype=a
http://en.scientificcommons.org/20885174

seropositivity in Medica sur (Mexico city)
http://new.medigraphic.com/cgi-bin/resumenMainI.cgi?IDARTICULO=392&IDPUBLICACION=99&IDREVISTA=17
in Mexican blood banks
http://gemi.mpl.ird.fr/PDF/Sanchez-Guillen.MIOC.2002.pdf
http://www.ajtmh.org/cgi/reprint/81/3/404.pdf

IMMUNOASSAYS

saliva elisa for dx chronic chagas

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T1R-3VJS2D9-4&_user=2488959&_coverDate=01%2F15%2F1999&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000057540&_version=1&_urlVersion=0&_userid=2488959&md5=a9374b978cf424c4d8cc6b607f389431&searchtype=a

http://www.ncbi.nlm.nih.gov/pubmed/9924959

http://jcm.asm.org/cgi/content/full/42/4/1766

SERUM

lateral flow immunoassay as alternative to riba (gold standard)
http://cvi.asm.org/cgi/content/full/16/4/515

new particle gel immunoassay
http://memorias.ioc.fiocruz.br/941/3620.pdf

what's used in US for screening blood since 2006
http://cat.inist.fr/?aModele=afficheN&cpsidt=20241826
basis for approval
http://www.fda.gov/downloads/BiologicsBloodVaccines/BloodBloodProducts/ApprovedProducts/LicensedProductsBLAs/BloodDonorScreening/InfectiousDisease/ucm089454.pdf

avoiding cross reactivity with leishmaniasis
http://www.bioline.org.br/request?oc97147

red cross procedures
http://www.redcrossblood.org/learn-about-blood/what-happens-donated-blood/blood-testing
A radioimmunoprecipitation assay (RIPA) is used in confirmatory testing. Because rates of recipient infection from transfusion are so low, the Red Cross qualifies each donor (rather than each donation) for negativity to antibodies to T. cruzi.

http://www.mayomedicallaboratories.com/test-catalog/Clinical+and+Interpretive/86159
http://mayomedicallaboratories.cws.net/test-catalog/print.php?unit_code=86159

antibody maturation in rats http://cvi.asm.org/cgi/content/full/8/4/802

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC86165/

a comparison
http://jmm.sgmjournals.org/cgi/content/full/55/2/171

http://www.arupconsult.com/Topics/ChagasDz.html
http://www.aruplab.com/guides/ug/tests/0051076.jsp IgG
http://www.aruplab.com/guides/ug/tests/0051075.jsp IgM

http://labs.unchealthcare.org/labstestinfo/t_tests/copy2_of_tissue_transiga.htm

http://www.scielo.cl/pdf/bres/v38n2-3/art08.pdf doesn't display on
school computesrs

mine this one for general info
http://memorias.ioc.fiocruz.br/941/3620.pdf

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